Mini Titrator for Measuring Formol Number in Wine & Fruit Juice

  • Part #:HA-HI84533
  • Weight:1.9 kg (67.0 oz.)
  • Availability: Inquire

Price: $1,256.44
Price: $1,696.19


This is a simple, fast, and affordable automatic mini titrator designed for testing formol number in wines or fruit juices. Based on an acid-base titration method, this mini titrator uses an optimized pre-programmed method of analysis with a powerful algorithm that determines the completion of the titration reaction by the use of a glass body pH electrode.  The unit incorporates a precision piston style dosing pump that adjusts the volume of dosing dynamically based on the voltage change. This dosing system reduces the amount of time required for the titration while providing for a highly accurate determination of the amount of titrant used.  This mini titrator is supplied complete with all the materials necessary to perform low and high range formol measurements. All chemicals are premixed and prepackaged including standardized titrants, reagents and pump calibration solution. There is no need for volumetric glassware or analytical balances.

The content of amino acids and other nitrogen compounds in fruit juices and wines is expressed as total assimilable nitrogen and is determined by the formol method using an acid-base titration. The formol number (also known as formol index) is a parameter used for evaluation of the quality of fruit juices and wines.

In wines, the concentration of alpha amino acid in grapes change as a function of maturity and crop load (yield to vine size ratio). The concentration increases with fruit maturation and decreases with crop load. In the fermentation of wine, there is a minimum amount of amino acid and other nitrogen compounds (e.g., 150 to 200 mg/L of yeast assimilable nitrogen) that has to be present in the must/juice. Too low of an amount will result in a stuck fermentation in which there is not enough nitrogen for the yeast to thrive. Because of the importance of nitrogen in fermentation, it is desirable to determine the nitrogen concentration before fermentation.

In fruit juices, the formol nitrogen number is one of the basic parameters measured to determine quality. Depending on the type of fruit, the number can increase or decrease with maturity. In orange and grapefruit juice, lower values are observed when the fruit is not suitably mature or there has been frost damage. In pineapple juice, a low number could be indicative of over-dilution with water or a disproportionate amount of the core was used. To determine the adulteration of fruit juices, the formol number, along with the chromatography characterization of amino acids, can be used.

This unit is supplied with a glass body pH electrode. This electrode is refillable and features a double junction, minimizing the contamination of the electrode. This versatile electrode has a spherical sensing glass bulb that provides a wide surface of contact with a wine or fruit juice sample. The unit uses a piston driven burette pump. This type of dosing system uses a motor in which each dose is very precisely controlled and the volume dispensed is accurately determined. The built-in stirrer is automatically maintained at a speed of 600 rpm, regardless of the viscosity of the solution being titrated.


(as N) Low Range: 2.14 to 28.57 meq/L; 0.21 to 2.85 meq%; 30.0 to 400.0 mg/L; High Range: 21.7 to 71.4meq/L; 2.14 to 7.14 meq%; 300 to 1000 mg/L
Low Range: 0.01 meq/L; 0.01 meq%; 0.1 mg/L; High Range: 0.1 meq/L; 0.01 meq%; 1 mg/L
Accuracy (@25ºC/77ºF)
+/- 0.1 mg/L or 3% of reading, whichever is greater
Sample Volume
Low Range: 10 mL; High Range: 5 mL
acid base titration
endpoint titration, adjustable (pH 8.0 - 8.5 in 0.1 increments)
Pump Speed
10 mL/min
Stirring Speed
600 rpm
pH Range
-2.0 to 16.0 pH; -2.00 to 16.00 pH
pH Resolution
0.1 pH / 0.01 pH
pH Accuracy (@25ºC/77ºF)
+/- 0.01 pH
pH Calibration
one, two, or three-point calibration, four available buffers (4.01, 7.01, 8.20, 10.01)
Temperature Compensation
manual or automatic
mV Range
-2000.0 to 2000.0 mV
mV Resolution
0.1 mV
mV Accuracy (@25ºC/77ºF)
+/- 1.0 mV
Temperature Range
-20.0 to 120.0°C; -4.0 to 248.0°F; 253.2 to 393.2 K
Temperature Resolution
0.1°C; 0.1°F; 0.1K
Temperature Accuracy (@25ºC/77ºF)+/- 0.4°C; ±0.8°F; ±0.4 K
Logging Data
up to 400 ( 200 titrations, 200 pH/mV readings)
HA-HI1131B glass body, refillable, with BNC connector and 1 m (3.3’) cable (included)
Temperature ProbeHA-HI7662-T stainless steel temperature probe with 1 m (3.3’) cable (included)
0 to 50°C (32 to 122°F); RH max 95% non-condensing
Power Supply
12 VDC adapter (included)
235 x 200 x 150 mm (9.2 x 7.9 x 5.9”)
1.9 kg (67.0 oz.)
Other information
HA-HI84533 is supplied with HA-HI1131B pH electrode, HA-HI7662-M temperature probe, HA-HI7082 electrode fill solution (30 mL), HA-HI84533-50 titrant solution (230 mL), HA-HI84533-55 pump calibration solution (120 mL), HA-HI84533-61 formol base reagent (230 mL), HA-HI84533-62 pH adjustment reagent (30 mL),HA- HI84533-60 hydrogen peroxide reagent (30 mL), 100 mL beakers (2), tube set (aspiration tube with titrant bottle cap and dispensing tube with tip), dosing pump valve, 5 mL syringe, 1 mL plastic pipette, cleaning solution sachets for wine deposits and wine stains (2), 12 VDC adapter, instruction manual and quality certificate.

Additional Information

Part #Description
Refillable Combination pH Electrode with BNC Connector for HA-HI84533 Tritrator
Replacement Stainless Steel Temperature Probe for HA-HI84533 Tritrator
HI 84533-50
Titrant for Formol Number Mini Titrator, 120 m
Pump Calibration Standard for Formol Number Mini Titrator, 120 ml
Formol Base Reagent for Formol Number Mini Titrator, 230 ml
pH Adjustment Reagent for Formol Number Mini Titrator, 30 ml
Hydrogen Peroxide Reagent for Formol Number Mini Titrator, 30 ml
Electrode Storage Solution (500 mL)
Electrolyte Solution 3.5M KCl (460 mL)
pH 4.01 Calibration Solution (500 mL)
pH 7.01 Calibration Solution (500 mL)
pH 8.20 Calibration Buffer Solution (230 mL)
pH 10.01 Calibration Solution (500 mL)
Cleaning Solution for Wine Deposits (500 mL)
Cleaning Solution for Wine Deposits (25 x 20 mL Sachets)
Cleaning Solution for Wine Stains (25 x 20 mL Sachets)