VRBG Agar + Neutralizing / Bile Esculin Agar + Neutralizing dipslide is a ready-to-use device with two different media coated onto a plastic support used for the microbial monitoring of surfaces and liquids even in the presence of residues of disinfectants. Violet Red Bile Glucose agar allows the isolation and enumeration of Enterobacteriaceae. Bile Esculin agar is used for the differentiation and isolation of enterococci (group D streptococci).

TEST PROCEDURE

1. Take a slide from the refrigerator and leave it at ambient temperature for about 5 minutes.
2. Unscrew and extract the slide from its cylindrical container. Avoid any contact with the agar surface.
3. For surface monitoring, flex the cap forming a 90° angle and press each side of the slide firmly against the surface to be examined for 10 seconds. Alternatively, use a swab for sampling the area, afterwards roll the swab gently over the agar surface. For examination of liquids, hold the slide by the cap and immerse it completely into the test fluid.
4. Reinsert the slide into its tube, screw it tight and incubate at 37 ± 1°C for 24-48 h. Note: different incubation temperatures and times may be required depending on the environment and organisms under investigation.

RESULTS INTERPRETATION

Enterobacteriaceae ferment glucose forming pink to red or purple colonies (with or without precipitation haloes) on VRBG Agar + Neutralizing (Side 1). On Bile Esculin Agar + Neutralizing (Side 2), a blackening of the medium around the colonies caused by esculin hydrolysis indicates the presence of enterococci.

APPEARANCE

Side 1. Very slightly to slightly opalescent, reddish-purple

Side 2. Slightly opalescent, dark-amber to olive green.

STORAGE CONDITIONS

10-25°C away from light, until the expiry date on the label. Eliminate if signs of deterioration or contamination are evident.

SHELF LIFE

9 months