TSA + Neutralizing / Rose Bengal Agar dipslide is a ready-to-use device with two different media coated onto a plastic support used for the microbial monitoring of surfaces and liquids even in the presence of residues of disinfectants. The selective medium allows the isolation and enumeration of yeasts and moulds; the other medium is used for enumeration of bacteria.

TEST PROCEDURE

1. Take a slide from the refrigerator and leave it at ambient temperature for about 5 minutes.
2. Unscrew and extract the slide from its cylindrical container. Avoid any contact with the agar surface.
3. For surface monitoring, flex the cap forming a 90° angle and press each side of the slide firmly against the surface to be examined for 10 seconds. Alternatively, use a swab for sampling the area, afterwards roll the swab gently over the agar surface. For examination of liquids, hold the slide by the cap and immerse it completely into the test fluid.
4. Reinsert the slide into its tube, screw it tight and incubate at 25 ± 1°C for up to 5 days.

RESULTS INTERPRETATION

Count the total number of colonies on TSA + Neutralizing (Side 1) to obtain the total bacterial count.

Total number of colonies grown on Rose Bengal Agar (Side 2) gives an assessment of the fungal contamination.

APPEARANCE

Side 1. Slightly opalescent, light amber.

Side 2. Slightly opalescent, bright pink.

STORAGE CONDITIONS

10-25°C away from light, until the expiry date on the label. Eliminate if signs of deterioration or contamination are evident.

SHELF LIFE

9 months