DG18 / DG18 dipslide is a ready-to-use device with a medium coated onto a plastic support used for the monitoring of microbial contamination. This medium is not intended for use in the diagnosis of disease or other conditions in humans.

The slide is covered on both sides with DG (Dichloran Glycerol) 18 agar which is used for selective isolation and enumeration of viable osmophilic yeasts and xerophilic moulds. This medium conforms to the performance and formulation requirements of ISO 21527-2. Neutralizing agents are also included allowing organisms to grow even in the presence of residues of disinfectants.

TEST PROCEDURE

1. Take a slide from the refrigerator and leave it at ambient temperature for about 5 minutes.
2. Unscrew and extract the slide from its cylindrical container. Avoid any contact with the agar surface.
3. For surface monitoring, flex the cap forming a 90° angle and press each side of the slide firmly against the surface to be examined for 10 seconds. Alternatively, use a swab for sampling the area, afterwards roll the swab gently over the agar surface. For examination of liquids, hold the slide by the cap and immerse it completely into the test fluid.
4. Reinsert the slide into its tube, screw it tight and incubate aerobically at 25 ± 1°C for 5 to 7 days. It is recommended to incubate for up to 10 days if the presence of Xeromyces bisporus is suspected.

RESULTS INTERPRETATION

Observe for fungal growth and count characteristic colonies/propagules after 2-day incubation and again at the end of the incubation period. Record the number of CFU per agar side.

The user is responsible for interpretation. It is recommended to establish alarm levels and levels that require user intervention, in order to take the most appropriate corrective action.

For surface testing, the colony count on each agar side can be calculated into CFU per cm² as follows: CFU (actual count) / 12.5 cm² = CFU/cm²

STORAGE CONDITIONS

10-25°C away from light, until the expiry date on the label. Eliminate if signs of deterioration or contamination are evident.

SHELF LIFE

9 months