Easy DryTM are absorbent pads saturated with a sterile, dehydrated culture medium. Each pad is pre-plated in a Petri dish and is immediately ready to use after pouring sterile distilled or deionized water on it. Easy DryTM are optimal for the examination of large sample volumes by the membrane filter method.
Easy DryTM Chromatic Salmonella is a selective and differential chromogenic medium used for the isolation and differentiation of Salmonella species in water, including drinking waters and samples from all stages of treatment and distribution.
This medium can be used as second isolation agar in the four-step procedure described in EN ISO 6579-1 for detection of Salmonella in food, animal feed and environmental samples.
PREPARATION
1.
Cut open a bag and remove the number of Easy Dry plates needed.
2.
Moisten the pad contained
in the Petri dish with 2.2 ml of sterile distilled or deionized water.
3.
Wait 5 minutes before
using.
TEST PROCEDURE
Filter the sample through a filter membrane (0.45 µm pore diameter). Transfer the membrane onto a plate containing a just rehydrated pad. Incubate aerobically at 35 ± 2°C for 18-24 hours.
INTERPRETING RESULTS
After incubation observe the color of the colonies and interpret the results as indicated in the ID table.
ID Table
|
Microorganism |
Typical colony color |
|
Salmonella spp (including S. Typhi, S. Paratyphi, lactose-positive,
sucrose-positive) |
Light
mauve to mauve |
|
E. coli, Enterobacter spp, Klebsiella spp |
Blue-green |
|
Other
organisms (if not inhibited) |
Colourless |
APPEARANCE OF THE MEDIUM
Whitish pad. Yellowish once rehydrated.
STORAGE
Store at 10-25°C away from light. Do not
use the product beyond its expiry date on the label or if product shows any
evidence of contamination or any sign of deterioration.
SHELF LIFE
2 years.
