Easy DryTM are absorbent pads saturated with a sterile, dehydrated culture medium. Each pad is pre-plated in a Petri dish and is immediately ready to use after pouring sterile distilled or deionized water on it. Easy DryTM are optimal for the examination of large sample volumes by the membrane filter method.
Easy DryTM Tergitol + TTC is a selective and differential medium used for the detection and enumeration of Escherichia coli and coliform bacteria in water.
PREPARATION
1.
Cut open a bag and remove the number of Easy Dry plates needed.
2.
Moisten the pad contained
in the Petri dish with 2.2 ml of sterile distilled or deionized water.
3.
Wait 5 minutes before
using.
TEST PROCEDURE
Filter two 100 ml aliquots of the water sample through two sterile membranes (0.45 µm pore diameter). Transfer each membrane onto a plate containing a just rehydrated pad. Incubate aerobically one plate at 37°C for 24 h (total coliforms) and the other one at 44°C for 16-24 h (Escherichia coli).
INTERPRETING RESULTS
Escherichia coli cultivates with yellow-orange colonies on yellow medium.
Enterobacter/Klebsiella cultivate with yellow colonies on yellow medium.
Salmonella/Shigella/Proteus/Pseudomonas cultivate with dark red colonies on green-blue medium.
APPEARANCE OF THE MEDIUM
Whitish pad. Pale green once rehydrated.
STORAGE
Store at 10-25°C away from light. Do not
use the product beyond its expiry date on the label or if product shows any
evidence of contamination or any sign of deterioration.
SHELF LIFE
2 years.
