Chromatic Coli Coliform / PCA + TTC + Neutralizing dipslide is a ready-to-use device with two media solidified onto a plastic support used for the microbial monitoring of surfaces of sanitary importance and analysis of food and water samples. The chromogenic and selective medium allows the detection of E. coli and the differentiation from the other enterobacteria. The other medium is used for enumeration of bacteria while inactivating disinfectants.

TEST PROCEDURE

1. Take a slide from the refrigerator and leave it at ambient temperature for about 5 minutes.
2. Unscrew and extract the slide from its cylindrical container. Avoid any contact with the agar surface.
3. For surface monitoring, flex the cap forming a 90° angle and press each side of the slide firmly against the surface to be examined for 10 seconds. Alternatively, use a swab for sampling the area, afterwards roll the swab gently over the agar surface. For examination of liquids, hold the slide by the cap and immerse it completely into the test fluid.
4. Reinsert the slide into its tube, screw it tight and incubate at 35 ± 2°C for 24-48 hours.

RESULTS INTERPRETATION

After incubation observe the color and the morphology of the colonies growth on Chromatic Coli Coliform (Side 1) and interpret the results as indicated in the ID table.

   ID Table

*β-glucuronidase-negative E. coli strains, such as E. coli O157, are colourless on this medium.

Count all the colonies on Plate Count Agar + TTC + Neutralizing (Side 2) and report as the number of colony- forming units (CFU) per sampled surface or CFU/ml for liquids.

APPEARANCE

Slightly opalescent, light amber on both sides.

STORAGE CONDITIONS

2-8°C away from light, until the expiry date on the label. Eliminate if signs of deterioration or contamination are evident.

SHELF LIFE

4 months